Stem Cell Therapy for Sperm Production?
Non-obstructive azoospermia involves a group of problems, which produce a similar outcome: Lack of sperm production/maturation. Spermatogenesis, which is a term used for the sum of all of the processes involved in production and maturation of a sperm cell, has multiple steps. Any malfunction during any of these steps can cause problems in obtaining a mature sperm cell in the ejaculate. These stages can be roughly categorized as Mitosis (making copies of the germ cells), meiosis 1, meiosis 2 and final differentiation. The whole process takes about 65-70 days to complete and results in a mature sperm cell.
Depending on the stage where sperm production/maturation comes to a halt is important for determining success with stem cell therapy. Sperm maturation problems could be attributed to intrinsic germ cell defects or extrinsic somatic and/or endocrine problems. Knowing the difference can be the key to identify the precise targeting of the problem. However, such targeted therapy is currently not available in the human model. The following picture helps with visualization of the spermatogenesis process:
Mitosis is the stage where the germ cell line multiples and grows in number. Differentiation comes in during the later stages. For instance, if spermatogenesis can reach the stage where it results in spermatids, then the main issue rests with final differentiation into the sperm cell as we know it. This is probably one of the most suitable cases for stem cell therapy as only one stage in the process of spermatogenesis is problematic and the likelihood of successfully addressing the problem is higher.
If the problem is in the earlier stages and there are no immature sperm cells being produced, then the task becomes more challenging. Therefore, the further down the process of spermatogenesis we run into problems, the higher the chance of success with any intervention we use.
"Sertoli cell only" syndrome refers to a condition where the testicular structures have nor germ cells and only sertoli cells are present. Sertoli cell only syndrome can be congenital or acquired. In congenital sertoli cell only syndrome, given that there are no germ cells present, there are currently no treatment modalities which can be offered. However, if the condition is acquired (klinefelter syndrome, mumps, radiation exposure to name a few), then the condition can be reversible and stem cell therapy can be administered.
As part of the stem cell therapy at North Cyprus IVF, we isolate stem cells from the patient’s own adipose tissue to be obtained from abdominal liposuction. This is a small-scale surgery, which is performed by a surgeon. Standard surgical procedures apply, which means food and drink restriction to be observed starting at a certain time before the surgery. We also perform a number of tests to confirm suitability for surgery. These are rather standard procedures.
Adipose tissue obtained via the liposuction process is then subjected to enzymal dissociation and several other procedures so that the stem cells can be successfully isolated. In the meanwhile, patient's platelet rich plasma (PRP) to be obtained from peripheral blood sample is also isolated separately. After the stem cells are subjected to culture media and seeding and once all the procedures are carried out, the combination of PRP with stem cell population is injected into testes at various different locations.
Afte the procedure, you are discharged from the hospital either on the same day or the next day, depending on recovery process. A semen analysis is required three months after the stem cell therapy to see the effects. In patients with intact spermatogenesis with the problems in later stages, we expect to observe live sperm cells in the ejaculate approximately three months after the procedure. If we do not observe live sperm cells in the ejaculate, a TESE/TESA procedure is scheduled to look for maturing sperm cells which can be used for fertilization. The entire treatment takes about 2-3 days (pre-testing, procedure itself and recovery time). This means you will only be required to stay in Cyprus for 2-3 days in total. The cost of this procedure is 6,000 Euros, which includes the pretests before surgery, all the laboratory work involved and injection of the stem cells as well as your stay at the hospital for one night. The tests required before planning your treatment are ideally done in your local country and are not included in this fee. You will find more information on our "Stem Cell Therapy for Non-Obstructive Azoospermia" page.
Please contact our patient coordinators for more information!
Associate Professor Dr. Savas Ozyigit, a pioneer in the field of assisted reproductive treatments in Cyprus, and the director of North Cyprus IVF Center receives a "Lifetime Achievement Award" for his contributions to the field of medicine over the course of his career. Adding one more trophy to his collection of many, Dr. Savas Ozyigit gave a short speech at his award acception ceremony.
During his speech, Prof. Ozyigit said "It was twenty years ago when I first mentioned it to colleagues that it was a shame couples with infertility problems could not be helped in North Cyprus and had to travel to other countries to seek help. People said it was not a good idea to invest in such a big project for such a small population. They said it would not be profitable and they said I might as well throw my money into a trash can. It wasn't the smallness of population that was the problem. It was the small faith they had in medicine. Today, I stand here holding an award for my work and accomplishment. This is the field of medicine thanking me for believing and taking that first step that no other person was willing to take just because they did not think it was financially viable. Not only it has been financially viable, it has allowed me to train many others to go into this field and become my competitors! There are now ten IVF clinics in North Cyprus! Thank you medicine, for being such a rewarding field. I would have chosen you all over if I were to come to this world again..."
Dr. Savas Ozyigit is the founder of North Cyprus IVF Center, the very first IVF clinic in Northern Cyprus, which was established in 1998. Almost all of the current IVF specialists in Northern Cyprus have been his students at one point during their medical training. As a medical innovator, he has always introduced many firsts in the field of medicine on the island. The first bone densitometry, the first mammogram, the first radical hysterectomy operation, the first IVF clinic and the first genetics laboratory are just a few examples.
We are sure that Dr. Savas Ozyigit still has many awards ahead of him. To contact North Cyprus IVF Center and schedule an appointment with Dr. Savas Ozyigit for your consult/fertility treatment, please use http://www.lowcostivf.net/contact.html or call/text/whatsapp +90 548 882 8000 / +90548 828 9955.
Dr. Savas Ozyigit
Egg donation in Cyprus, Gender selection in Cyprus and IVF treatments in North Cyprus in general: you will find information on new advances in IVF technology and latest IVF practices. All the blogs are personally written by Dr. Savas Ozyigit and his expert medical team.